1. Husbandry
Breeding Systems
Maximum avoidence of inbreeding is used for stock maintenance system infoundation stock and random mating system is used for production in production stock.
Environment
The Mlac:ICR is housed in controlled environment. The ventilation, temperature and humidity are controlled by HVAC (Heating, Ventilating, Air conditioning) system.
– Temperature range is controlled at about 22 ± 2 ํC
– Relative humidity range at about 55 + 10%
– Air changes per hour at 15-20 ACH
– Light: dark cycle is adjusted at 12:12 hours
– Mouse are sensitive to noise so the noise level should be maintained at< 85 db.
Feed and water
The Mlac:ICR is fed on the commercial diet (No. 082) product of Perfect companion group co. Ltd., the animal feed manufacturer which milled according to our requested specifications. Food is provided in stainless steel wire- bar lid feeders ad libitum. The RO Water is used for the drinking water which is chlorinated at a concentration of 5-6 ppm to make it free from Pseudomonas aeruginosa. The drinking water is provided in bottles with sipper tubes.
Bedding
Dried water hyacinth and corn cob are is used for mouse as bedding material. The same bedding is used in shipping containers. Dried bedding is packed in 3 Layers paper bags and sterilized by autoclave at 135 ํC for 7 minutes before used.
Equipment
Different size of cages are used in different breeding processes as follows.
Dimensions of Enclosure (W×L×H)(centimeter) | Maximum Number Animals/Enclosure | Types of Enclosure & Description |
---|---|---|
7.5 × 11.5 × 5 | 6 animals/cage (or) 1 animal (lactating female)/cage | Polysulfonate and stainless steel cages with stainless steel wire-bar lids |
11 × 18× 6 | 4 Female + litter (lactating female)/cage | Stainless steel shoe box type with stainless wire-bar lid |
14 × 29× 6 | 8 Female + litter (lactating female)/cage | Stainless steel shoe box type with stainless wire-bar lid |
14 × 29× 6 | 35 animals(5-8 weeks)/cage 50 animals(3-4 weeks)/cage |
Stainless steel shoe box type with stainless wire-bar lid |
Space requirement for Mlac:ICR.
Weight, g. | Floor Area/Animal,(in2) | Height/inches |
---|---|---|
Under 10 | 6 | 5 |
Up to 15 | 8 | 5 |
Up to 25 | 12 | 5 |
Over 25 | At least 15 | 5 |
Click here for more information of “Inbred Strain / Outbred Stock”